Hangzhou, ChinaMon–Fri 9:00–18:00 (UTC+8)zcsw2017@163.com
For Research Use Only. Not for use in diagnostic procedures.
ZC BIOSCIENCE
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Isothermal nucleic acid detection

Rapid isothermal nucleic acid detection with RAA

Recombinase-Aided Amplification (RAA) delivers a fluorescence result in 5–15 minutes at a constant 37–42 ℃. No thermal cycler. Lyophilised reagents and a portable analyzer bring molecular testing to the field.

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Gloved hand holding an 8-tube strip of lyophilised RAA reagent pellets
Lyophilised RAA reagent strip
5–15 minTime to fluorescence result
37–42 ℃Constant reaction temperature — no thermal cycling
1 %Limit of detection, meat adulteration (w/w)
103 cfu/mLLimit of detection, foodborne pathogen kits
RAA technology

One enzyme system. One temperature. Minutes to result.

Recombinase-Aided Amplification (RAA) is a rapid isothermal nucleic acid amplification technology. A recombinase obtained from bacteria or fungi binds tightly to the primer DNA at ambient temperature, forming a recombinase–primer complex. When the primer locates its fully complementary sequence on the template, single-stranded DNA-binding protein helps open the double strand, and DNA polymerase synthesises a new complementary strand. The product accumulates exponentially.

With a labelled fluorescent probe the reaction is read in real time; a fluorescence result is typically available within 5–15 minutes. All reaction components are pre-mixed and lyophilised in the tube — add Buffer A, Buffer B and the extracted sample DNA. RAA can in principle be multiplexed: two or more primer pairs in one reaction amplify several targets simultaneously. RAA is the isothermal amplification platform behind every ZC Bioscience reagent; all kits are developed and manufactured in-house.

01

Simple operation

Molecular-level testing that does not require specialist staff. Two-step workflow.

02

Fast

Results in 5–15 minutes; truly rapid testing.

03

Accurate

High sensitivity and specificity; precise recognition of the target sequence.

04

Portable

Compact instruments suited to harsh field conditions and on-site inspection.

05

Isothermal

Runs at 30–42 ℃; no thermal cycler or other expensive equipment required.

06

Lyophilised

Reagents supplied as freeze-dried pellets, ready to use, easy to ship, and free from freeze–thaw loss.

Platform comparison

RAA vs PCR vs LAMP

TechnologyRAAPCRLAMP
EnzymesRecombinase, single-stranded DNA-binding protein, strand-displacing DNA polymeraseTaq DNA polymeraseStrand-displacing DNA polymerase
Reaction temperature37–39 ℃, constantHigh temperature, thermal cycling60–65 ℃
Reaction time15 min1.5 h40 min
Primer pairs112
Reagent formatLyophilised pelletLiquidLiquid
ReadoutGel electrophoresis; real-time fluorescence; lateral-flow stripGel electrophoresis; real-time fluorescenceVisual turbidity
Consideration—Slow; thermal cycler requiredAerosol carry-over; false positives

Typical values as documented for each platform. RAA reagents are for research use only.

Products

Reagents, extraction and instruments for one workflow

Five product lines built around RAA: core amplification enzyme kits for assay developers, a portable fluorescence analyzer, rapid extraction kits, a food-safety molecular detection series, and a fully automated sample-in/result-out system. All reagents are for research use only (RUO). Pricing on request.

RAA core amplification kits (enzyme reagents) for building your own isothermal assay. Rapid — 30 min at 30–42 ℃ (optimum 37–39 ℃) yields a gel-detectable product. Specific — the recombinase–primer complex recognises the target by base pairing. Sensitive — over 107-fold amplification. Primers should be 30–35 bp and probes 46–52 bp for optimal performance.

Six kits · 48 tests/kit · 50 μL reactionLyophilised pellets. Store at −20 ℃, dry, protected from light; 12 months.
8-tube strip of lyophilised RAA reagent
Cat. No. S001ZC

RAA Basic Nucleic Acid Amplification Kit (RUO)

DNA, gel readout. Amplifies the target at 37 ℃; product confirmed by agarose gel electrophoresis. Heat block, water bath or incubator.

DNAGel readout48 tests
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8-tube strip of lyophilised RAA reagent
Cat. No. S002ZC

RAA Fluorescent Nucleic Acid Amplification Kit (RUO)

DNA, real-time. Real-time monitoring on the Genchek analyzer or standard real-time PCR instruments; 5–20 min.

DNAReal-time fluorescence48 tests
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8-tube strip of lyophilised RAA reagent
Cat. No. S003ZC

RT-RAA Basic Nucleic Acid Amplification Kit (RUO)

RNA, gel readout. RNA is first reverse-transcribed to cDNA; 37 ℃ isothermal amplification with gel readout.

RNAGel readout48 tests
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8-tube strip of lyophilised RAA reagent
Cat. No. S004ZC

RT-RAA Fluorescent Nucleic Acid Amplification Kit (RUO)

RNA, real-time. Reverse transcription followed by real-time RAA on Genchek or standard qPCR instruments; 5–20 min.

RNAReal-time fluorescence48 tests
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8-tube strip of lyophilised RAA reagent
Cat. No. S005ZC

RAA-nfo Nucleic Acid Amplification Kit, Lateral-Flow Strip (RUO)

DNA. 39 ℃ isothermal amplification; dual-labelled amplicon read on a universal nucleic acid lateral-flow strip.

DNALateral-flow strip48 tests
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8-tube strip of lyophilised RAA reagent
Cat. No. S006ZC

RT-RAA-nfo Nucleic Acid Amplification Kit, Lateral-Flow Strip (RUO)

RNA. 42 ℃ isothermal amplification with reverse transcription; strip readout.

RNALateral-flow strip48 tests
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An integrated, portable rapid-test analyzer for on-site and mobile testing. Built-in reagent mixing, fully matched to RAA reagents; dual fluorescence channels (FAM & ROX); automatic data analysis and report generation with multiple report-export options. All assays run under one software interface and results are read from a single display.

Genchek Isothermal Fluorescence Analyzer8 wells × 2 channels — up to 16 targets per run.
Genchek isothermal fluorescence analyzer, white benchtop unit
Instrument

Genchek Isothermal Fluorescence Analyzer

Throughput — 8 wells × 2 channels (FAM & ROX). Easy — simple operation, quick to learn. Real-time — live fluorescence curves. Smart — automatic analysis and report generation, multiple export routes.

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Specifications

Technical data

Throughput
8 wells
Fluorescence channels
FAM / ROX
Temperature range
Ambient to 65 ℃
Temperature accuracy
±0.5 ℃
Net weight
3.8 kg
Dimensions (L×W×H)
255 × 240 × 123 mm
Power
120 W · AC 100–240 V, 50/60 Hz
Operating system
OPENRTOS
Operating environment
5–35 ℃, 20–80 % RH
Storage environment
−20 to 55 ℃, <90 % RH

Compatible reagents also run on standard real-time PCR instruments (ABI, MJ, Roche, Bioer series).

Purpose-built extraction chemistries that yield RAA-ready nucleic acid in minutes. No hazardous solvents, low cross-contamination risk. Store at room temperature, dry; 12 months.

Four kits · heat lysis, spin column, magnetic bead5 to 25 minutes from sample to template.
Gloved hand holding an 8-tube reagent strip
Cat. No. ND01ZC

Rapid DNA Extraction Reagent, Heat-Lysis (RUO)

Genomic DNA in 5 min from animal tissue, blood, meat products, plant tissue, seeds and bacteria. No protein or RNA removal steps, no organic extraction, no ethanol precipitation.

Heat lysis5 min50 preps
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8-tube strip
Cat. No. ND02ZC

Universal Rapid DNA Extraction Kit, Spin Column (RUO)

Lyse–bind–wash–elute in 15 min. Animal tissue, cells, bacteria, saliva, blood, swabs, faeces, bone, feed; high yield and quality.

Spin column15 min50 preps
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8-tube strip
Cat. No. ND03ZC

Universal Rapid RNA Extraction Kit, Spin Column (RUO)

Four steps, 10 min, high-quality RNA from animal tissue, cells, saliva, blood, swabs and faeces.

Spin column10 min50 preps
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8-tube strip
Cat. No. ND06ZC

Magnetic-Bead DNA Extraction Pre-filled Plate (RUO)

For automated magnetic-bead extractors. Nano-magnetic-bead purification with high yield and consistency; cuts hands-on time for large sample batches in food safety, environmental microbiology, livestock and molecular biology research.

Magnetic bead25 min96 preps
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Ready-to-run RAA fluorescent kits for food-safety laboratories. Isothermal at 30–42 ℃ (optimum 37–39 ℃); result read directly from the amplification curve — no electrophoresis. Compatible with Genchek and standard real-time PCR instruments (ABI, MJ, Roche, Bioer series). 24 tests/kit. Store at −20 ℃, protected from light; 6 months.

Three groups · 17 catalogued kits plus GMO element kitsAnimal species · foodborne pathogens · GMO screening.
Group A · 10 kits

Animal-Derived Ingredient Molecular Detection Kits (RAA, Fluorescent) (RUO)

Species-specific gene fragment; detects different breeds of the target species with no cross-reaction observed against common species. Limit of detection 1 % (w/w). For animal tissue, blood, foods, meat products (incl. processed meat) and feed.

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  • Chicken-Derived Ingredient Kit (RUO)A101ZC
  • Duck-Derived Ingredient Kit (RUO)A102ZC
  • Pork-Derived Ingredient Kit (RUO)A103ZC
  • Bovine-Derived Ingredient Kit (RUO)A104ZC
  • Ovine/Caprine-Derived Ingredient Kit (RUO)A105ZC
  • Rat-Derived Ingredient Kit (RUO)A106ZC
  • Horse-Derived Ingredient Kit (RUO)A107ZC
  • Mink-Derived Ingredient Kit (RUO)A108ZC
  • Fox-Derived Ingredient Kit (RUO)A109ZC
  • Donkey-Derived Ingredient Kit (RUO)A110ZC
24 tests/kitLOD 1 % (w/w)Result 5–15 min
Group B · 7 kits

Foodborne Pathogen Molecular Detection Kits (RAA, Fluorescent) (RUO)

Recognises and amplifies a specific gene fragment of the target organism; result in 5–15 min; limit of detection 103 cfu/mL. Qualitative detection in foods, beverages, water, cosmetics, hygiene products and seafood. Method reference: GB 4789 series, SN/T series.

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  • Staphylococcus aureus Kit (RUO)B201ZC
  • Salmonella Kit (RUO)B202ZC
  • Pseudomonas aeruginosa Kit (RUO)B203ZC
  • Listeria monocytogenes Kit (RUO)B204ZC
  • Haemolytic Streptococcus Kit (RUO)B205ZC
  • Vibrio parahaemolyticus Kit (RUO)B206ZC
  • Shigella Kit (RUO)B207ZC
  • E. coli O157:H7 and Vibrio cholerae Kits (RUO)On request
24 tests/kitLOD 103 cfu/mLResult 5–15 min
Group C · GMO screening

GMO Molecular Detection Kits for Agricultural Products (RAA, Fluorescent) (RUO)

Specifically detects exogenous elements and events in crops, identifying GM material at the molecular level. Six screening elements plus event-specific kits for soybean, maize and canola.

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  • CaMV 35S Promoter Detection Kit (RUO)Element
  • FMV 35S Promoter Detection Kit (RUO)Element
  • nos Terminator Detection Kit (RUO)Element
  • Bt Gene Detection Kit (RUO)Element
  • bar Gene Detection Kit (RUO)Element
  • hpt Gene Detection Kit (RUO)Element
  • GM Soybean GTS 40-3-2 / MON89788 Event Kits (RUO)Event
  • GM Maize MON810 / Bt11 · GM Canola T45 / Oxy-235 Event Kits (RUO)Event
24 tests/kitReal-time fluorescenceResult 5–15 min

Fully automated, sample-in/result-out in 30 minutes. Performs pipetting, lysis, nucleic acid purification, isothermal amplification, fluorescence detection, analysis and reporting in one instrument. Load the sample and the system does the rest.

Genchek Fully Automated Isothermal Nucleic Acid Detection SystemExtraction and amplification integrated; ~30 min total.
Blue DNA double helix illustration
Instrument

Genchek Fully Automated Isothermal Nucleic Acid Detection System

Fast — extraction and amplification integrated, 30 min total. Hands-off — automated extraction, purification, amplification and detection. Real-time detection. Smart reporting with multiple export routes.

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Specifications

Technical data

Sample throughput
1
Run time
~30 min
Channels
FAM / ROX
Interface
7-inch colour touchscreen
UV lamp · air purification
Yes · yes
Weight
16 kg
Dimensions (L×W×H)
260 × 325 × 420 mm
Power
221 W · AC 220 V, 50 Hz
Pipetting
<15 μL: accuracy >98 %, CV <5 % · 15–50 μL: >98.5 %, CV <3 % · >50 μL: >99 %, CV <1.2 %
Operating environment
20–30 ℃, 20–80 % RH
Solutions

Three food-safety questions, one 15-minute answer

Isothermal RAA reagents specifically recognise and amplify the target gene fragment; the Genchek analyzer monitors amplification in real time and the run completes in 15 minutes. Technology: RAA (Recombinase-Aided Amplification). Instrument: Genchek fluorescence analyzer.

DNA helix illustration with two laboratory scientists

Meat species authentication

As price gaps between meats widen, species substitution and adulteration are increasingly common. Short shelf lives and high sample volumes call for a fast, accurate and practical method. RAA-based species kits detect species-specific genes, identifying meat origin at the molecular level with no cross-reaction between species.

Time to result
5–15 min
Sensitivity
1 % adulterated meat
Format
Lyophilised
  • Chicken
  • Pork
  • Duck
  • Beef
  • Sheep/goat
  • Rat
  • Horse
  • Mink
  • Fox
  • Donkey

Users — regulators: food and drug administration, customs and border inspection, livestock authorities, agricultural research institutes, law enforcement. Industry: food manufacturers, dietary-supplement and feed producers, third-party testing laboratories.

Laboratory technician transferring a sample into a petri dish inside a biosafety cabinet

Foodborne pathogen detection

In China, pathogenic bacteria account for an estimated 40–50 % of reported foodborne-illness cases each year. ZC Bioscience's RAA pathogen kits combine multi-channel detection, short run times and high sensitivity — raising testing throughput, moving food-safety warnings earlier and cutting labour cost. Qualitative detection in food, beverages, cosmetics and seafood.

Time to result
5–15 min
Limit of detection
103 cfu/mL
Method reference
GB 4789 / SN/T series
  • S. aureus
  • Salmonella
  • P. aeruginosa
  • L. monocytogenes
  • Haemolytic Streptococcus
  • V. parahaemolyticus
  • Shigella
  • E. coli O157:H7
  • V. cholerae

Users — regulators: food and drug administration, customs and border inspection, public-health laboratories. Industry: food manufacturers, cosmetics manufacturers, third-party testing laboratories.

Blue DNA double helix illustration

GMO screening

GM crops carry genes introduced from other organisms to alter traits, nutrition or quality. Alongside their economic value they raise questions of food safety and environmental impact, and regulators worldwide require labelling and control to protect consumers' right to know. RAA-based GMO kits specifically detect exogenous elements and events in crops.

Time to result
5–15 min
Readout
Real-time fluorescence
Format
Lyophilised
  • CaMV 35S promoter
  • FMV 35S promoter
  • nos terminator
  • Bt
  • bar
  • hpt

Users — regulators: food and drug administration, customs and border inspection, agricultural research institutes. Industry: food manufacturers, feed producers, third-party testing laboratories.

Services

Support from first question to custom target

Our R&D team answers every product question with patient explanation and demonstration, and develops custom RAA assays on request.

Technical service

Expert technical support

Our R&D team answers every product question with patient explanation and demonstration. We provide consultation, technical documentation, training and after-sales support.

  • Application consultation
  • Technical documentation and IFU
  • Operator training
After-sales

After-sales service

Good after-sales service earns customer trust. We provide quality after-sales service with long-term technical documentation and support. If a problem arises, our engineers respond promptly and resolve issues remotely by e-mail or phone.

  • Tel +86 571 8861 9668
  • zcsw2017@163.com
  • Mon–Fri 9:00–18:00 (UTC+8)
Custom development

Custom assay development

RAA enables portable, rapid nucleic acid testing across foodborne pathogen detection, meat adulteration, GMO screening and more. We develop custom RAA assays to meet specific customer needs — contact us to discuss your target.

  • Target-specific RAA assay design
  • OEM reagent supply
  • Co-development under strategic agreements
About ZC Bioscience

R&D-driven, built around RAA

Hangzhou ZC Bio-Sci & Tech Co., Ltd. ("ZC Bioscience") is an R&D-driven biotechnology company based in Hangzhou, China. We develop and apply Recombinase-Aided Amplification (RAA), an isothermal nucleic acid amplification technology, and supply RAA-based reagents, extraction kits and portable fluorescence instruments for rapid molecular testing.

Since September 2016 the company has accelerated product development for RAA-based rapid food-safety testing, launching in turn the animal-species (meat authentication) molecular detection series, the foodborne pathogen molecular detection series and the GMO ingredient molecular detection series. Other application areas include environmental monitoring, aquaculture pathogen detection and animal disease detection. ZC Bioscience has signed strategic cooperation agreements with government institutions and research organisations across several fields to co-develop RAA-based detection kits.

500 m²Laboratory space, plus 400 m² of offices
RMB 12M+Installed equipment value
90 %Of the R&D team hold master's degrees or higher
5050Hangzhou Binjiang District "5050 Talent Program" selection
Technician working at a biosafety cabinet
Biosafety cabinet
Laboratory with benches and instruments
R&D laboratory
Clean-room corridor with observation window
Clean-room corridor
Technician working at a laminar-flow clean bench
Clean bench
News

Company news

Explainer | Recombinase-Aided Rapid Nucleic Acid Amplification

Nucleic acid amplification lets trace nucleic acid be amplified rapidly in vitro. RAA replaces the thermal cycling of conventional PCR with recombinase, single-stranded DNA-binding protein and DNA polymerase, achieving rapid amplification at a constant 37 ℃.

Read more

Literature | Rapid Nucleic Acid Amplification by Recombinase-Aided Amplification

In vitro rapid amplification enables efficient amplification of trace nucleic acids and has been widely applied in molecular biology since its inception. Recombinase-aided amplification is an isothermal method developed on the basis of existing amplification principles.

Read more

Highlights | RAA Technology Debuts at the Food Microbiology Detection & Control Exchange

On 20–22 July 2017 the 7th Food Microbiology Detection and Control Technology Exchange was held in Shanghai, with 17 themed sessions, over 30 exhibitors and 300+ laboratory professionals attending.

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Forum | 7th Food Microbiology Detection & Control Technology Exchange (19–22 July)

Foodborne disease caused by pathogenic microorganisms is the number-one food-safety issue. Controlling contamination and strengthening laboratory capability have become focal concerns for regulators and food companies alike.

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ZC Bioscience at the 10th China International Food Safety & Quality Conference (CBIFS 2017)

The annual CBIFS food-safety technology forum was held on 7–8 April 2017 at the Hangzhou International Expo Center. ZC Bioscience, a newcomer to food-safety testing, made its debut at the conference.

Read more

Have a target in mind?

Tell us the organism, species or element and the sample type. We will come back with a kit recommendation and a quotation.

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Contact

Talk to the team

Distributor enquiries, quotations and custom assay requests. We reply in English within two working days.

Company
Hangzhou ZC Bio-Sci&Tech Co. Ltd
Address
Tianhe Hi-Tech Park, Bldg 5, 688 Bin'an Rd, Binjiang District, Hangzhou 310052, China
Hours
Mon–Fri 9:00–18:00 (UTC+8)
By submitting you agree to be contacted about your enquiry. All reagents are for research use only.